Shandong Provincial Inspection Center for Medical Devices/Key Lab of Biological Evaluation of Medical Devices of Shandong
Province, Jinan Shandong 250101, China
Abstract:Objective By comparing the hemostasis time, bleeding volume and local tissue reaction of two groups of collagen
hemostatic sponge and common gauze in the liver trauma model of pigs, an effective bleeding wound model was established for
the evaluation of absorbable hemostatic materials in animal experiments. At the same time, it provides reference for the clinical
application of collagen hemostatic sponge. Methods Eighteen experimental miniature pigs (female without pregnant, body weight
30~40 kg) were randomly divided into three groups (group A: collagen sponge group; group B: medical collagen sponge group;
group C: normal gauze group). A model of hepatic hemorrhage was established in each pig. The hemostatic time and amount of
bleeding of collagen hemostatic sponge and gauze were measured quantitatively. After 8 weeks, the formation of blood capsule
of wound was observed in anatomical animals, and HE staining was used to observe the inflammatory reaction and wound
healing in the injured liver tissue. Results The hemostasis time of group A, B and C were (215.00±69.50), (205.00±48.06) and
(480.00±90.99) s, respectively. The hemorrhage volume was (3.08 +1.65), (2.71 +1.34) and (15.77 +3.58) g, respectively. There
were significant statistical differences in hemostasis time and hemorrhage volume among group A, B and group C (P<0.05). The
pathological observation showed that there was no inflammatory reaction in all animal liver wounds, no bloody capsule was found,
and the wound healed well. Conclusion Porcine liver hemorrhage model can effectively evaluate the effectiveness of hemostatic
materials in this model. Collagen hemostatic sponge has a significant hemostatic effect compared with the normal gauze.