荧光成像技术在组织热损伤评价中的应用

doi:10.3969/j.issn.1674-1633.2016.03.011

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摘要目前组织热损伤主要依靠离体切片组织学进行评价，尚没有一种较理想的评价方法。本研究设计了一种利用340 nm波长的自体荧光成像技术评估活体组织热损伤的方法，并采用该方法对激光照射后的牛腱组织进行了荧光成像，将荧光成像后的牛腱组织样本与日光灯下成像后的牛腱组织样本通过天狼星红F3BA染色的组织学方法进行了验证和比较。实验结果显示，样本中受激光照射区域的胶原蛋白的自体荧光发生了明显改变，说明该方法能够用于评价组织热损伤。

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	汤陌生

关键词 ：荧光成像技术, 组织热损伤, 胶原蛋白

Abstract：Currently thermal tissue damage is mainly evaluated with histological examination which requires the removal of a section of tissue.There is no ideal method to detect molecular changes after thermal damage tissue. The research designed a new method to evaluate thermal tissue damage in situ using native fluorescence spectroscopic imaging under 340 nm excitation. Native fluorescence spectroscopic imaging was performed on laser irradiated bovine tendon tissue. The fluorescence imaging of the tendon tissue were confirmed and compared with the image of the laser-irradiated tendon tissue with the histological method of picrosirius red F3BA stain. The results of the experiment indicate that the changes in native fluorescence from collagen on the laser-irradiated tissue could be observed on the thermal damage region of the tissue.

Key words： fluorescence imaging tissue thermal damage collagen

收稿日期: 2015-11-04

R445.2

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引用本文:

汤陌生. 荧光成像技术在组织热损伤评价中的应用[J]. 中国医疗设备, 2016, 31(3): 53-55.
TANG Mo-sheng. Application of Fluorescence Imaging in the Evaluation of Thermal Damage to Tissue. China Medical Devices, 2016, 31(3): 53-55.

链接本文:

http://cs.china-cmd.org/zgylsb/CN/10.3969/j.issn.1674-1633.2016.03.011 或 http://cs.china-cmd.org/zgylsb/CN/Y2016/V31/I3/53

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	Surg Med,1994,15(2):168-175.
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	in temperature response to the sinusoidal heating at theskin
	Surg Med,1994,15(2):168-175.
	Surg Med,1994,15(2):168-175.
[3]	Liu J,Xu XL.Estimation of blood perfusion using phaseshift
	in temperature response to the sinusoidal heating at theskin
	surface[J].IEEE Trans Biomed Eng,1999,46(9):1037-1043.
[4]	Davies CR,Saidel GM,Harasaki H.Sensitivity analysis ofonedimensional
[4]	Davies CR,Saidel GM,Harasaki H.Sensitivity analysis ofonedimensional
	heat transfer in tissue with temperaturedependent
	heat transfer in tissue with temperaturedependent
	perfusion[J].J Biomech Eng,1997,119(1):77-80.
[5]	赵友全,范世福,李小霞.生物组织光热传输和热损伤的研究[J].
[5]	赵友全,范世福,李小霞.生物组织光热传输和热损伤的研究[J].
	中国激光,2004,31(5):631-634.
	激光生物学报,2006,15(1):97-100.
[2]	Maitinot VJ,Mordon SR,Mitchell VA,et al.Determination of
	rats: macroscopic, thermal,and histological evaluation[J].Lasers
[1]	张先增,谢树森,林慧韫.生物组织激光消融阈值的光谱特性[J].
	激光生物学报,2006,15(1):97-100.
[2]	Maitinot VJ,Mordon SR,Mitchell VA,et al.Determination of
	efficient parameters for argon laser-assisted anastomoses in
	rats: macroscopic, thermal,and histological evaluation[J].Lasers
	surface[J].IEEE Trans Biomed Eng,1999,46(9):1037-1043.