Establishment of a Rabbit Model for Selective Ablation of Inferior Vena Cava Vascular
Smooth Muscle Cells Using High-Voltage Ultrashort Electric Field Pulses
WANG Xiaohui1a, YUAN Zhenpeng1a, HONG Liangjie2, WU Bin2, CHEN Xinhua1b,2, WANG Bing1a, WU Ziheng1a
1. a. Division of Vascular Surgery; b. Division of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang
University School of Medicine, Hangzhou Zhejiang 310003, China;
2. Zhejiang Provincial Key Laboratory of Pulsed Power Translational Medicine, Hangzhou Zhejiang 311100, China
Abstract：Objective High-voltage ultra-short electric field pulse has a biological effect of not destroying the intact structure of
cells and inducing autonomous apoptosis of cells through instantaneous and high-energy local electric field changes, which has
potential application value in inhibiting vascular remodeling, vessel wall proliferation and vascular stenosis. In this study, a new type
of high-voltage ultra-short electric field pulse system was independently developed to explore the biological effects of the system
on rabbit inferior vena cava. Methods The inferior vena cava (IVC) of experimental rabbits was stimulated by 1000 V/cm, 1 Hz
high voltage ultra-short electric field pulse for 60 times. After routine feeding, the IVC specimens of experimental rabbits were
dissected and separated after death on the third day after surgery, and the specimens were sent to HE pathological staining for routine
examination. Results The self-developed new high-voltage ultra-short electric field pulse system was successfully prepared. The
smooth muscle was apoptosis, the vascular wall became thinner, and the local vascular endothelial cells had no obvious injury. In the
control area without electrical stimulation, the vascular endothelial cells, smooth muscle cells and vascular wall structure were intact.
Conclusion High pressure ultra-short electric field pulse can selectively ablation smooth muscle cells and maintain the integrity of
peripheral vascular endothelium, smooth muscle cells and tissue structure.
王晓辉1a，苑振鹏1a，洪良杰2，吴斌2，陈新华1b,2，王冰1a，吴子衡1a. 高压超短电场脉冲高选择性兔下腔静脉
血管平滑肌细胞消融模型建立的探索[J]. 中国医疗设备, 2023, 38(5): 15-19.
WANG Xiaohui1a, YUAN Zhenpeng1a, HONG Liangjie2, WU Bin2, CHEN Xinhua1b,2, WANG Bing1a, WU Ziheng1a. Establishment of a Rabbit Model for Selective Ablation of Inferior Vena Cava Vascular
Smooth Muscle Cells Using High-Voltage Ultrashort Electric Field Pulses. China Medical Devices, 2023, 38(5): 15-19.
