安徽医科大学 a. 生物医学工程学院;b. 3D打印与组织工程中心 安徽省转化医学研究院,安徽 合肥 230032
Review of Development of Diffraction Phase Microscopy
WANG Penga, PENG Taoa, WU Haoa, LU Fengyaa, ZHONG Zhenshenga, WANG Yia, ZHOU Jinhuaa,b
a. School of Biomedical Engineering; b. 3D-Printing and Tissue Engineering Center, Anhui Provincial Institute of Translational
Medicine, Anhui Medical University, Hefei Anhui 230032, China
Abstract:Quantitative phase imaging (QPI) as unlabeled microscopic imaging, has become one of the most valuable methods in
biomedical applications. Diffraction phase microscopy (DPM) is a high-sensitivity QPI method based on slightly off-axis commonpath
structure, which has the characteristics of label-free, high stability and low-noise phase images. This paper systematically
introduced the theory of interference imaging, the principles of selection of system parameters, and the phase retrieval algorithm.
Moreover, the developments of DPM method was summarized, including white-light diffraction phase microscopy, multi-wavelength
diffraction phase imaging, structural light illumination diffraction phase imaging, and multi-mode diffraction phase imaging.
Additionally, the application of these developed DPM methods in biology and medicine is introduced. Finally, the limitations of the
DPM methods and the possible development in the future were also discussed.
汪鹏a,彭韬a,吴昊a,陆凤雅a,钟振声a,王奕a,周金华a,b. 衍射相位显微成像的发展综述[J]. 中国医疗设备, 2023, 38(4): 19-24.
WANG Penga, PENG Taoa, WU Haoa, LU Fengyaa, ZHONG Zhenshenga, WANG Yia, ZHOU Jinhuaa,b. Review of Development of Diffraction Phase Microscopy. China Medical Devices, 2023, 38(4): 19-24.