安徽医科大学 a. 生物医学工程学院；b. D打印与组织工程中心 安徽省转化医学研究院，安徽 合肥 230032
Design of Living Cell Workstation Based on PLC
SUN Yuea, KE Zeyua, WANG Zixina, ZHOU Jinhuaa,b, ZHANG Shengzhaoa,b
a. School of Biomedical Engineering; b. 3D-Printing and Tissue Engineering Center, Anhui Provincial Institute of Translational
Medicine, Anhui Medical University, Hefei Anhui 230032, China
Abstract：Objective Live cell real-time imaging technology is widely used in the biomedical field and is an important tool for
studying cell physiological state and cell activities. In this paper, a living cell workstation based on programmable logic controller is
designed, which can realize long-time monitoring and real-time imaging of living cells. Methods With S7-200 SMART as the main
controller, the operation of heating and ventilation devices could be controlled by reading the internal environmental parameters of the
living cell workstation, so as to provide appropriate temperature, humidity and CO2 concentration for cells. The real-time imaging of
cells was achieved based on the microscope in the station, and the data could be monitored and stored in real time through LabVIEW.
Results This paper produced a living cell workstation, and the stability of the key parameters was tested. The results showed that the
device had good stability in controlling temperature (steady state error: 0.1℃), relative humidity (steady state error: 0.2%) and CO2
concentration (steady state error: 0.2%). The control stability was good, and the real-time imaging function of the device was verified
by cell culture test. Conclusion The device has the function of traditional living cell workstation, and has the advantages of simple
operation and low cost, which provides convenience for biomedical research and clinical application.
孙越a，柯泽宇a，汪子歆a，周金华a,b，张盛昭a,b. 基于PLC的活细胞工作站的设计[J]. 中国医疗设备, 2023, 38(4): 13-18.
SUN Yuea, KE Zeyua, WANG Zixina, ZHOU Jinhuaa,b, ZHANG Shengzhaoa,b. Design of Living Cell Workstation Based on PLC. China Medical Devices, 2023, 38(4): 13-18.
