Abstract：Objective To investigate the effects of receptor for advanced glycation end products (RAGE) receptor blocker on EEG,
neuron injury and S100B protein expression in epileptic young rats. Methods Forty specific pathogen free grade male SD rats
were randomly divided into normal group (group N ), model group (group M), phenytoin sodium group (group P) and RAGE
receptor blocker group (group R), with 10 rats in each group. The epilepsy model was established by intraperitoneal injection of
pentylenetetrazol in group M, group P and group R, but not in group N. After successful modeling, 20 mg/kg phenytoin sodium was
injected intraperitoneally in P group, 1 mg/kg RAGE receptor blocker FPS-ZM was injected intraperitoneally in group R, and the
same volume of 0.9% sodium chloride solution was injected intraperitoneally in group N and group M. The behavior of the young
rats in each group was observed, the EEG was recorded, the neuronal injury damage was detected by FJB staining and Nissl staining,
and the expression of S100 B protein was detected by immunoenzymatic labeling. Results Compared with group N, the Racine
score, seizure duration and seizure times of young rats in group M, group P, group R were significantly increased (P<0.05); compared
with group M, the Racine score, seizure duration and seizure times of young rats in group P and group R were significantly decreased
(P<0.05); and compared with group P, above index in the group R were significantly decreased (P<0.05). Compared with group
N, the amplitude of wave in group M, group P, group R was significantly increased (P<0.05), and the frequency was significantly
decreased (P<0.05); compared with group M, the amplitude of wave in group P and group R was significantly decreased (P<0.05),
and the frequency was significantly increased (P<0.05); and compared with group P, group R was significantly changed (P<0.05).
Compared with group M, the neuronal injury in group P and group R was significantly reduced (P＜0.05), the number of neurons
increased (P＜0.05), the morphology of neurons in group P and group R was improved (P<0.05), the number of neurons and Nissl
bodies was significantly increased. Compared with group N, the expression of S100 B protein in hippocampus, midbrain and frontal
cortex in group M, group P and group R were significantly increased (P<0.05); compared with group M, the expression of S100B
protein in hippocampus, midbrain and frontal cortex of group P and group R were significantly decreased (P<0.05); and compared
with group P, above index in group R was significantly decreased (P<0.05). Conclusion RAGE receptor blockers can significantly
improve EEG and neuronal injury, and inhibit S100B protein expression in epileptic young rats.
姜小花，陈利娟，陈敏，耿立建. RAGE受体阻断剂对癫痫幼鼠脑电图、
神经元损伤及S100B蛋白表达的影响[J]. 中国医疗设备, 2023, 38(5): 156-160.
JIANG Xiaohua, CHEN Lijuan, CHEN Min, GENG Lijian. Effects of RAGE Receptor Blocker on EEG, Neuron Injury and S100B Protein Expression in
Epileptic Young Rats. China Medical Devices, 2023, 38(5): 156-160.
