Evaluation of Renal Blood Perfusion in Early Resuscitation of Rabbit Model of Hemorrhagic
Shock by Contrast-enhanced Ultrasonography and the Correlation with Renal Function
HE Jingling1, LIN Xian1, LONG Chunyan2, WEI Fang2, LI Yekuo2
1. Department of Ultrasound Imaging, Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou Guangdong
510120, China; 2. Department of Cardio Thoracic Surgery, General Hospital of Southern Theater Command, Guangzhou Guangdong
510010, China
Abstract：Objective To quantitatively analyze the changes in renal blood perfusion during early resuscitation of hemorrhagic shock
(HS) by contrast-enhanced ultrasound (CEUS). Methods Thirty-two healthy experimental rabbits were randomly divided into
4 groups, 1 group was randomly selected as the normal control group, the other three groups were used to establish the HS model
according to the Wiggers modified method, of them, 1 group was used as the shock group, the remaining 2 groups were divided into 2 h
resuscitation group and 6 h resuscitation group according to the different resuscitation time. CEUS was used to observe the rabbits’
renal perfusion and the perfusion parameters were recorded including amplitude of peak intensity (A), time to peak (TTP), area under
the curve (AUC) and gradient between start frame to peak frame (Grad). The correlation between the above parameters and renal
function and pathological results was analyzed. Results Compared with the normal control group, the TTP of the shock group had
prolonged, the A, AUC and Grad decreased, the differences were statistically significant (P<0.05), the HS model was successfully
established; Creatinine (Cr) and blood urea nitrogen (BUN) increased, which suggested that kidney function was impaired. In the
2 h resuscitation group, the A , AUC and Grad increased; TTP, BUN and Cr decreased slightly compared with the shock group, the
difference was statistically significant (P<0.05), which indicated that the parameters gradually recovered after fluid resuscitation.
However, compared with the normal control group, TTP, Grad, BUN and Cr were still statistically different (P<0.05). Compared the
6 h resuscitation group with the shock group, all parameters were statistically different (P<0.05), compared with the normal control
group, the parameters were not significantly different, and the correlation between AUC and laboratory indicators (BUN and Cr)
was the highest, which was consistent with the pathological results. Conclusion CEUS can quantitatively analyze the changes in
renal cortex blood perfusion in the early stage of resuscitation from HS. AUC can be used as a sensitive indicator and has a good
correlation with renal function indicators. CEUS can be used as a non-invasive monitoring method.
何晶玲1，林娴1，龙春艳2，魏芳2，李叶阔2. 超声造影评价兔失血性休克复苏早期肾血流灌注及与肾功能的相关性[J]. 中国医疗设备, 2022, 37(4): 140-144.
HE Jingling1, LIN Xian1, LONG Chunyan2, WEI Fang2, LI Yekuo2. Evaluation of Renal Blood Perfusion in Early Resuscitation of Rabbit Model of Hemorrhagic
Shock by Contrast-enhanced Ultrasonography and the Correlation with Renal Function. China Medical Devices, 2022, 37(4): 140-144.
