Preparation and in Vitro Study of Fluorescence Magnetic Resonance Bimodal Molecular
Probes Based on Carboxyl Graphene Quantum Dots
LI Zhongtao1, CHEN Song2a, HAO Liguo2b, GUO Jing1, GU Hongqian2b, LIU Qiang1, WANG Li3, WANG Lei2b, LI Guohua1
1. Department of Imaging, The First Affiliated Hospital of Qiqihar Medical College, Qiqihar Heilongjiang 161041, China;
2. a. Laboratory of Pharmaceutical Chemistry, School of Pharmacy; b. Molecular Imaging Laboratory, Institute of Medical
Technology, Qiqihar Medical University, Qiqihar Heilongjiang 161000, China; 3. Radiology Center, The Third Affiliated Hospital of
Qiqihar Medical University, Qiqihar Heilongjiang 161000, China
Abstract：Objective To construct a bimodal fluorescence-magnetic resonance molecular probe based on carboxyl fossilized ink
quantum dots (Gd@GQDs) and to investigate its properties and application in cell imaging. Methods In 1-(3-dimethylaminopropyl)-
3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) catalyzed the combination of diamino polyethylene glycol
(2000) with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA). Nh2-peg-dota-peg-nh2 was connected to the surface
of graphene quantum dots (GQDs), and finally Gd3+ was chelated into DOTA to synthesize Gd@GQDs, and its size, fluorescence
performance and cytotoxicity were detected. Results Gd@GQDs transmission electron microscopy showed that the
particle size was 31.58±11.82 nm. The hydrated particle size was (39.7±21.5) nm. The mass fraction of Gd3+ in the probe was
2.46%. Conclusion The probe has good biocompatibility, wide excitation wavelength and high relaxation rate, which is of great
significance for cell imaging research.
李忠涛1，陈颂2a，郝利国2b，郭靖1，谷弘谦2b，刘强1，王丽3，王雷2b，李国华1. 以羧基化石墨烯量子点为载体的荧光-磁共振双模态分子探针的制备及体外研究[J]. 中国医疗设备, 2022, 37(5): 64-68.
LI Zhongtao1, CHEN Song2a, HAO Liguo2b, GUO Jing1, GU Hongqian2b, LIU Qiang1, WANG Li3, WANG Lei2b, LI Guohua1. Preparation and in Vitro Study of Fluorescence Magnetic Resonance Bimodal Molecular
Probes Based on Carboxyl Graphene Quantum Dots. China Medical Devices, 2022, 37(5): 64-68.
