尤斯灌流室系统应用的研究进展

doi:10.3969/j.issn.1674-1633.2016.11.018

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摘要尤斯灌流室（Ussing Chamber）由丹麦学者Ussing研究NaCl主动转运时首次介绍于世，其简单实用性使其在生物医学界受到越来越多的关注。Ussing Chamber为研究离子、营养物质、药物在各种上皮组织的转运提供了一个生理系统，其主要是通过微电极来检测整个细胞膜的跨膜电位差、短路电流、跨膜电阻。随着科技的不断进步，Ussing Chamber的用途也越来越广泛。本文结合国内外学者对其研究进行了简单综述，简要介绍其基本结构、原理、应用及未来发展趋势。

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	陈立峰^1a
	董晗²
	郑骏^1a
	郑天怡^1a
	宋文锋^1b

关键词 ： Ussing Chamber, 胃肠道屏障功能, 营养运输, 药物吸收, 胎盘屏障

Abstract：The Ussing Chamber was developed by the Danish biologist Ussing as a mean to understand the phenomenon of active NaCl transport, which had attracted more and more attention among the biomedical industry for its simplicity and practicability. The Ussing Chamber provided a physiological system to measure the transport of ions, nutrients and drugs across various epithelial tissues. It measured the trans-epithelial potential, short-circuit current and trans-epithelial resistance of the plasm membrane through microelectrodes. With the progress of science and technology, the Ussing Chamber had become more and more widely applied. This paper reviewed the researches about Ussing Chamber from national and international studies in recent years, and introduced the design, structure, principle, and its application in the field of biomedicine, as well as its application and development trend.

Key words： Ussing chamber gastrointestinal barrier function nutrition transport drug absorption placental barrier

收稿日期: 2015-11-10

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引用本文:

陈立峰^1a，董晗²，郑骏^1a，郑天怡^1a，宋文锋^1b. 尤斯灌流室系统应用的研究进展[J]. 中国医疗设备, 2016, 31(11): 73-77.
CHEN Li-feng^1a, DONG Han²,ZHENG Jun^1a, ZHENG Tian-yi^1a,SONG Wen-feng^1b. Progress in Research on the Application of Ussing Chamber Progress in Research on the Application of Ussing Chamber. China Medical Devices, 2016, 31(11): 73-77.

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http://cs.china-cmd.org/zgylsb/CN/10.3969/j.issn.1674-1633.2016.11.018 或 http://cs.china-cmd.org/zgylsb/CN/Y2016/V31/I11/73

	Acta Physiol Scand,1951,23:110-127.
[2]	Clarke LL.A guide to Ussing Chamber studies of mouse intestine[J].
	肠外与肠内营养,2006,13(4):233-236.
	Acta Physiol Scand,1951,23:110-127.
	Acta Physiol Scand,1951,23:110-127.
[1]	Ussing HH,Zerahn K.Active transport of sodium as the sourceof electrical current in the short-circuited isolated frog skin[J].
	Am J Physiol Gastrointest Liver Physiol,2009,296(6):G1151-G1166.
[1]	Ussing HH,Zerahn K.Active transport of sodium as the sourceof electrical current in the short-circuited isolated frog skin[J].
[2]	Clarke LL.A guide to Ussing Chamber studies of mouse intestine[J].
[3]	Le Ferrec E,Chesne C,Artusson P,et al.In Vitro Models of the
[3]	Le Ferrec E,Chesne C,Artusson P,et al.In Vitro Models of the
[4]	杨振,秦环龙.Ussing Chamber在肠道屏障功能研究中的进展[J].
[4]	杨振,秦环龙.Ussing Chamber在肠道屏障功能研究中的进展[J].
	肠外与肠内营养,2006,13(4):233-236.
[3]	Le Ferrec E,Chesne C,Artusson P,et al.In Vitro Models of the
	intestinal barrier[J].Altern Lab Anim,2001,29:649-668.
	肠外与肠内营养,2006,13(4):233-236.
[1]	Ussing HH,Zerahn K.Active transport of sodium as the sourceof electrical current in the short-circuited isolated frog skin[J].
[2]	Clarke LL.A guide to Ussing Chamber studies of mouse intestine[J].
	Am J Physiol Gastrointest Liver Physiol,2009,296(6):G1151-G1166.
	Am J Physiol Gastrointest Liver Physiol,2009,296(6):G1151-G1166.
	intestinal barrier[J].Altern Lab Anim,2001,29:649-668.
	intestinal barrier[J].Altern Lab Anim,2001,29:649-668.
[4]	杨振,秦环龙.Ussing Chamber在肠道屏障功能研究中的进展[J].