Abstract：Objective To explore the mechanism of intravenous immunoglobulin (IVIg), glucocorticoids (GCs) and human umbilical
cord mesenchymal stem cells (hUC-MSCs) on related cytokines by establishing mouse model of Kawasaki disease (KD), to
provide new therapeutic scheme for the treatment of KD. Methods Mice were divided into normal group, KD group, IVIg group,
GCs group and hUC-MSCs group according to random number method, with 10 mice in each group. KD model was established
in all groups except the normal group. After successful modeling, IVIg group was injected with IVIg, GCs group was injected
with methylprednisolone sodium succinate injection, hUC-MSCs group was injected with hUC-MSCs 1×106, and KD group was
injected with 0.9% NaCl solution. The weight of mice in each group was observed, and the coronary artery diameter was examined
by high-resolution small animal ultrasound. Serum thrombin (TB), fibrinogen (FIB), D-dimer (DD), thromboxane B2 (TXB2) and
inflammatory factors were detected by ELISA. Tissue lesions were observed by HE staining and electron microscopy. The protein
of nuclear transcription factor kappa B (NF-κB) and matrix metalloproteinase-9 (MMP-9) and the number of positive cells were
detected by immunostaining and immunohistochemistry. Results The body weight of IVIg group, GCs group and hUC-MSCs
group was higher than that of normal group at on 1st, 7th, 14th and 28th day and the inner diameter thickness of coronary artery was
lower than that of KD group (all P<0.05). Compared with normal group, KD group mice showed increased TB, FIB, DD, TXB2,
tumor necrosis factor-α, interleukin (IL)-6 and IL-1β (P<0.05). Compared with KD group, the above indexes in IVIg group, GCs
group and hUC-MSCs group were all significantly decreased (P<0.05). Compared with GCs group, the above indexes in hUCMSCs
group significantly were decreased (P<0.05). The results of HE staining and electron microscopy showed that the coronary
artery tissue structure was intact and the inner elastic membrane was continuous in normal group; the arrangement of artery tissue was disordered and a large number of endothelial cells were shed in KD group; the inflammatory infiltration of IVIg group, GCs
group and hUC-MSCs group was reduced, and endothelial cell vacuole was improved. Compared with normal group, there were
significant differences in the increase of NF-κB and MMP-9 protein and the number of positive cells in KD mice (P<0.05). Compared
with KD group, the above indexes in IVIg group, GCs group and hUC-MSCs group were significantly decreased (P<0.05). Compared with
GCs group, the above indexes in hUC-MSCs group were significantly decreased (P<0.05). Conclusion All the three methods can reduce
the serum TB, FIB, DD and TXB2 levels in KD mice, and have strong anti-inflammatory effects, increase the weight of mice and inhibit
the coronary artery diameter thickness, especially hUC-MSCs, which may be related to the inhibition of NF-κB and MMP-9 activities.
赵欣，赵颖，张雪梅，金晶. 不同治疗方案对川崎病小鼠模型相关细胞
因子的作用机制研究[J]. 中国医疗设备, 2023, 38(7): 130-138.
ZHAO Xin, ZHAO Ying, ZHANG Xuemei, JIN Jing. Study on the Mechanism of Different Treatment Regimens on Related Cytokines in
Kawasaki Disease Mouse Model. China Medical Devices, 2023, 38(7): 130-138.
