Effects of Oxygen Enrichment Intervention on Cognitive Function and Morphology
of Hippocampus CA1 Region of Rats Under Acute Hypobaric Hypoxia Environment
RUAN Junyong1,2, LEI Tao1, DONG Xu1, ZHAI Mingming1, LUO Erping1
1. Department of Teaching and Research for Army Health Equipment, Military Biomedical Engineering School, Air Force Military
Medical University, Xi’an Shaanxi 710032, China; 2. Department of Equipment, Qingdao First Sanatorium of PLA Navy, Qingdao
Shandong 266071, China
Abstract：Objective To explore the effects of oxygen enrichment intervention by portable membrane oxygen machine on cognitive
function and morphology of hippocampus CA1 region of rats under a simulated acute hypobaric hypoxia environment, and provide
a theoretical basis for the application and evaluation of oxygen machine. Methods Thirty male SD rats were randomly divided
into three groups: NC group, LO group and HO group. A simulated altitude of 6000 m acute hypobaric hypoxia environment was
provided by the environment simulation cabin for small animal experiment. SD rats in the LO and HO groups were exposed to
hypobaric hypoxia environment for 3 days, whereas SD rats in the HO group were enriched with oxygen for 12 h during the daytime,
and SD rats in the NC group were exposed to air. The cognitive function of rats was determined by Morris water maze test, and
HE staining in hippocampus CA1 region was observed under light microscope. Results Compared with rats in the NC group and
the HO group, rats in the LO group presented significantly decreased number of platform crossings (P<0.05), while there was no
significant difference between the NC group and the HO group. The degenerative pyramidal cells in the LO group were significantly
higher than that in both the NC and the HO groups (P<0.05). The structural damage of the hippocampal pyramidal cells in the HO
group was significantly alleviated relative to that in the LO group. There was no significant difference in the number of degenerative
pyramidal cells between the HO and NC groups (P>0.05). Conclusion Under the simulated altitude of 6000 m acute hypobaric
hypoxia environment, oxygen enrichment intervention using a portable membrane oxygen oxygen machine can effectively alleviate
hippocampal damage and significantly improve the cognitive function of rats.
阮俊勇1,2，雷涛1，董旭1，翟明明1，罗二平1. 急性低压低氧环境下富氧干预对大鼠认知功能及海马CA1区形态学的影响[J]. 中国医疗设备, 2018, 33(12): 39-41.
RUAN Junyong1,2, LEI Tao1, DONG Xu1, ZHAI Mingming1, LUO Erping1. Effects of Oxygen Enrichment Intervention on Cognitive Function and Morphology
of Hippocampus CA1 Region of Rats Under Acute Hypobaric Hypoxia Environment. China Medical Devices, 2018, 33(12): 39-41.