Method for Characterizing the Porous Structure of Absorbable Collagen Materials
WANG Changbin1,2,3, WANG Xiuxiu1,2,3, CHEN Hongjian1,2,3, AI Chongchong1,2,3, WAN Min1,2,3, LU Wenbo1,2,3
1. Shandong Institute of Medical Device and Pharmaceutical Packaging Inspection, Jinan Shandong 250101, China; 2. NMPA Key
Laboratory for Safety Evaluation of Biomaterials and Medical Devices, Jinan Shandong 250101, China; 3. Shandong Key Laboratory of
Biological Evaluation for Medical Devices, Jinan Shandong 250101, China
Abstract：Objective To develop a method for characterizing the microstructure of absorbable collagen materials, and analyze their
porosity and pore size. Methods Field emission scanning electron microscope (SEM) was used, images of non-gold-sprayed samples
and gold-sprayed samples were captured at different acceleration voltages. The obtained SEM images were analyzed for porosity and
pore size by using Image J software, and the availability of the results was verified. Results The clear SEM images were obtained by
using a lower acceleration voltage for non-gold-sprayed samples. After gold spraying, the ability to withstand voltage was significantly
improved, and the clear SEM images could be obtained even at higher acceleration voltages. The obtained SEM images were analyzed for
pore size and porosity using Image J software. Among them, the average porosity was 50.9% and the average pore size was 11.9 μm. The
dispersion coefficient of porosity was 8.6%. and the dispersion coefficient of pore size was 6.7%. Conclusion The combination of field
emission SEM and Image J software is an effective method for characterizing the porous structure of absorbable collagen materials,
which can solve the issue of missing methods in relevant standards.
王常斌1,2,3，王秀秀1,2,3，陈洪建1,2,3，艾冲冲1,2,3，万敏1,2,3，卢文博1,2,3. 一种表征可吸收胶原蛋白材料孔结构
的方法研究[J]. 中国医疗设备, 2024, 39(4): 8-12.
WANG Changbin1,2,3, WANG Xiuxiu1,2,3, CHEN Hongjian1,2,3, AI Chongchong1,2,3, WAN Min1,2,3, LU Wenbo1,2,3. Method for Characterizing the Porous Structure of Absorbable Collagen Materials. China Medical Devices, 2024, 39(4): 8-12.
