安徽医科大学 a. 生物医学工程学院；b. 3D打印与组织工程中心 安徽省转化医学研究院，安徽 合肥 230032
Portable Multimode Microscope for Morphological Classification of Unstained Red Blood Cells
KE Zeyua, SHAO Menga, PENG Taoa, ZHANG Cana, WANG Zixina, FANG Shua, ZHOU Jinhuaa,b
a. School of Biomedical Engineering; b. 3D-Printing and Tissue Engineering Center, Anhui Provincial Institute of Translational
Medicine, Anhui Medical University, Hefei Anhui 230032, China
Abstract：Objective To develop a portable multimode microscope for detecting the morphology of red blood cells (RBCs), and to
provide a new idea for the morphological classification of unstained RBCs. Methods The microscope components were fabricated
by 3D printing technology. Three imaging modes, including bright field, dark field, and oblique lighting were realized by using
encoded LED array illumination. The grayscale distribution of single RBC under the three modes was analyzed to distinguish the
morphological difference of unstained RBCs. Results The spatial resolution of the device was higher than 2.19 μm, which was close
to that of commercial microscope; for the stored RBC samples diluted 150 times, the self-developed microscope combined with
oblique lighting, dark field, and bright field imaging could effectively distinguish the morphology of echinocytes, spherocytes, and
discocytes, while it could not be determined by commercial microscope; the difference of luminance distribution between spherocytes
and discocytes in bright and dark field might be used as a new methodology of RBC classification. Conclusion The microscope is
simple, portable, and cost-effective, which can be applied to detect the morphology of unstained RBCs. It has potential practicability
in remote areas, outdoor first aid, and wartime medical treatment.
