Establishment and Evaluation of Molecular Probe Verification Model in
Brain Tumor Specific Imaging
HUO Tian-long1, YANG Shuo1,
LI Tian-ran2, KANG Yu1,
ZHAO Yun-yun1, DU Xiang-ke1
1.Department of Radiology, Peking
University People’s Hospital, Beijing
100044, China; 2.Department of Radiology,
the 95th Hospital of PLA, Putian
Fujian 351100, China
Abstract：Objective To establish a dualtumor
animal model in one nude mouse which positively and
negatively expresses a specific receptor respectively. Methods The human hepatocellular carcinoma
cell line BEL-7402 (positively expressed SSTR2) and the human lung cancer cell line A-549 (negatively
expressed SSTR2) were selected and cultured respectively. Then cells in logarithmic growth phase
were digested and suspended for transplantation to the bilateral axillary fossa of the same nude mouse
(the concentration of the cells suspended were over 5×106/0.2 mL), so as to establish the dual-tumor
epidermal xenograft nude mouse model. The effects of the tumor transplantation were observed and
analyzed. Results The two kinds of cell lines were successfully cultured; the BEL7402/
A549 dualtumor
nude mouse xenograft model was successfully established in the bilateral axillary fossa of one nude
mouse. The tumor formation rate of BEL-7402 cell line was above 80% (24/30), and it was easy to form
the tumor if the cell number was large enough. Whereas the tumor formation rate of A549 cell line was
relatively low, which was about 73% (22/30) over a concentration of 5×106/0.2 mL, and the success rate
of tumor formation could be increased via cells augmentation or secondary injection. The number of the
bilateral tumor whose size difference was within 1 cm was accounted for 32% (8/22). The histological
examination showed that the hepatocellular carcinomas dissected from the tumorbearing
nude mice
were typically malignant form compared with normal mice liver, the cell nucleus was large, pathologic
mitosis were commonly seen with dual or multi nucleus. The obvious necrosis and abundant vessels
were seen in tumors which suggested that the tumor grew fast and the microvessels
were rich. Although
the general form of the A549 transplantation tumors were different from the BEL7402
transplantation
tumors, the micromorphology
form of the two tumors were consistent with the corresponding tumors of
human beings. Conclusion The different tumor of cell lines could be inoculated to the same nude mouse
to successfully develop the dualtumor
models. To wellcontrol
the growth difference of the two kinds
of tumors could establish the size-proper tumor models, so as to lay a foundation for tumor specificity imaging.
霍天龙1，杨硕1，李天然2，康钰1，赵赟赟1，杜湘珂1. 肿瘤特异性成像中分子探针验证模型的建立及评估[J]. 中国医疗设备, 2017, 32(3): 10-13.
HUO Tian-long1, YANG Shuo1,
LI Tian-ran2, KANG Yu1,
ZHAO Yun-yun1, DU Xiang-ke1. Establishment and Evaluation of Molecular Probe Verification Model in
Brain Tumor Specific Imaging. China Medical Devices, 2017, 32(3): 10-13.
